PEPTIDE
RECONSTITUTION
CALCULATOR

PEPTIDE
RECONSTITUTION
CALCULATOR

PEPTIDE
RECONSTITUTION
CALCULATOR

Calculate how much bacteriostatic water to add to your peptide vial and determine the concentration per ml.

Calculate how much bacteriostatic water to add to your peptide vial and determine the concentration per ml.

Calculate how much bacteriostatic water to add to your peptide vial and determine the concentration per ml.

mg

Common sizes: 2mg, 5mg, 10mg

ml

Common amounts: 1ml, 2ml, 3ml

mcg

Example: 250mcg for BPC-157, 2500mcg for TB-500

RECONSTITUTION CALCULATOR

About The Peptide Reconstitution Calculator

About The Peptide Reconstitution Calculator

Calculate exact concentration and injection volumes when mixing peptides


Reconstituting peptides means mixing freeze-dried peptide powder with bacteriostatic water.


The math determines your concentration (mcg/ml) and tells you exactly how much to draw in your syringe for each dose.


Get the concentration wrong and every dose is off. This calculator does the math instantly - enter your peptide amount (mg), water amount (ml), and target dose (mcg). Get your exact injection volume.


How peptide reconstitution works:

You receive peptides as lyophilized powder in a vial. Before you can inject, you must add bacteriostatic water. The amount of water you add determines concentration.


Example: 5mg peptide + 2ml water = 2,500mcg/ml concentration


If you need 250mcg per dose: 250mcg ÷ 2,500mcg/ml = 0.10ml to inject

More water = lower concentration = larger injection volumes. Less water = higher concentration = smaller injection volumes. Neither is better - choose based on your target dose size and syringe precision.

HOW

How to Reconstitute Peptides

How to Reconstitute Peptides

Step-by-step mixing guide for peptide powder

Step-by-step mixing guide for peptide powder

What you need:

  • Peptide vial (powder form)

  • Bacteriostatic water (0.9% benzyl alcohol)

  • 3ml syringe for drawing water

  • Alcohol swabs

  • Refrigerator for storage


Reconstitution process:

Step 1: Remove plastic cap from peptide vial. Wipe rubber stopper with alcohol swab, let dry 10 seconds.


Step 2: Draw your chosen water amount into a 3ml syringe (typically 1-3ml).


Common amounts:

  • 1ml for high concentration (smaller injections)

  • 2ml for medium concentration (balanced)

  • 3ml for low concentration (easier to measure small doses)


Step 3: CRITICAL TECHNIQUE - Aim needle at the inside wall of the vial, NOT directly at the powder. Inject water slowly down the glass wall. This prevents damage to peptide chains.


Step 4: DO NOT SHAKE. Let vial sit for 2-3 minutes. Powder dissolves on its own. Gentle swirling in circular motion is okay if needed.


Step 5: Solution should be clear. Cloudiness = contamination or damaged peptide. Don't use cloudy solutions.


Step 6: Refrigerate immediately at 2-8°C (36-46°F). Write reconstitution date on vial. Use within 2-4 weeks.


Why bacteriostatic water, not sterile water?

Bacteriostatic water contains benzyl alcohol preservative that prevents bacterial growth.


You're drawing multiple doses from one vial over weeks. Sterile water lacks preservative - bacteria can grow between uses.


Never use tap water or distilled drinking water. Not sterile. Will contaminate and destroy your peptide.

FREE TOOLS

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Tools to optimize every aspect of your peptide protocol

Tools to optimize every aspect of your peptide protocol

FAQ

Peptide reconstitution questions answered

Peptide reconstitution questions answered

The process of properly mixing your peptides

The process of properly mixing your peptides

What is peptide reconstitution and why is it necessary?

Reconstitution is the process of adding bacteriostatic water to lyophilized (freeze-dried) peptide powder to create an injectable solution. Peptides are shipped as powder because they're more stable in this form. You must reconstitute them before use.

What is peptide reconstitution and why is it necessary?

Reconstitution is the process of adding bacteriostatic water to lyophilized (freeze-dried) peptide powder to create an injectable solution. Peptides are shipped as powder because they're more stable in this form. You must reconstitute them before use.

What is peptide reconstitution and why is it necessary?

Reconstitution is the process of adding bacteriostatic water to lyophilized (freeze-dried) peptide powder to create an injectable solution. Peptides are shipped as powder because they're more stable in this form. You must reconstitute them before use.

Can I use regular sterile water instead of bacteriostatic water?

Can I use regular sterile water instead of bacteriostatic water?

Can I use regular sterile water instead of bacteriostatic water?

How much bacteriostatic water should I add to my peptide vial?

How much bacteriostatic water should I add to my peptide vial?

How much bacteriostatic water should I add to my peptide vial?

How do I properly reconstitute peptides without damaging them?

How do I properly reconstitute peptides without damaging them?

How do I properly reconstitute peptides without damaging them?

How long do reconstituted peptides last in the refrigerator?

How long do reconstituted peptides last in the refrigerator?

How long do reconstituted peptides last in the refrigerator?

What does concentration mean and how does it affect my injections?

What does concentration mean and how does it affect my injections?

What does concentration mean and how does it affect my injections?

Can I reconstitute multiple vials at once to save time?

Can I reconstitute multiple vials at once to save time?

Can I reconstitute multiple vials at once to save time?

Why is my reconstituted peptide cloudy or has particles?

Why is my reconstituted peptide cloudy or has particles?

Why is my reconstituted peptide cloudy or has particles?

Ready to optimize your peptide use?

Ready to optimize your peptide use?

Know you're doing it safely, save hundreds on wrong peptides, and finally see the results you've been working for

Know you're doing it safely, save hundreds on wrong peptides, and finally see the results you've been working for